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验证数据展示

  • WB analysis of MCF-7 using 11069-2-AP

    WB analysis of MCF-7 using 11069-2-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11069-2-AP (FMR1NB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 11069-2-AP

    IHC staining of mouse testis using 11069-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11069-2-AP (FMR1NB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FMR1NB Polyclonal antibody

FMR1NB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11069-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Cancer/testis antigen 37, CT37, FMR1NB, NY SAR 35, Sarcoma antigen NY SAR 35

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, A549 cells, MCF-7 cells, SKOV-3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11069-2-AP targets FMR1NB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FMR1NB fusion protein Ag1531 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fragile X mental retardation 1 neighbor
别名 Cancer/testis antigen 37, CT37, FMR1NB, NY SAR 35, Sarcoma antigen NY SAR 35
计算分子量 29 kDa
观测分子量 66 kDa
GenBank蛋白编号BC034320
基因名称 FMR1NB
Gene ID (NCBI) 158521
RRIDAB_2105567
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N0W7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FMR1NB antibody 11069-2-APDownload protocol
IHC protocol for FMR1NB antibody 11069-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols