• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human brain using 11244-1-AP

    WB analysis of human brain using 11244-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11244-1-AP (AASDHPPT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 11244-1-AP

    IF Staining of HeLa using 11244-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AASDHPPT antibody (11244-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

AASDHPPT Polyclonal antibody

AASDHPPT Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 11244-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

CGI-80, CGI 80, Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase, AASD-PPT, AASD PPT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11244-1-AP targets AASDHPPT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 AASDHPPT fusion protein Ag1763 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
别名 CGI-80, CGI 80, Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase, AASD-PPT, AASD PPT
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC016728
基因名称 AASDHPPT
Gene ID (NCBI) 60496
RRIDAB_2304862
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRN7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AASDHPPT, also known as L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase, is required for mitochondrial respiration and oxidative metabolism via the mitochondrial fatty acid synthesis (mtFAS) pathway. AASDHPPT catalyzes the post-translational modification of target proteins by phosphopantetheine and transfer the 4'-phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, including the acyl carrier domain of FASN.

实验方案

Product Specific Protocols
WB protocol for AASDHPPT antibody 11244-1-APDownload protocol
IF protocol for AASDHPPT antibody 11244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

bioRxiv

Mitochondrial Phosphopantetheinylation is Required for Oxidative Function

Authors - Pieter R Norden
View Article