验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Jurkat cells, MCF7 cells, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human stomach cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
11254-1-AP targets GLRX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GLRX3 fusion protein Ag1775 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutaredoxin 3 |
| 别名 | GLRX3, GLRX4, Glutaredoxin 3, GRX3, GRX4, PICOT, PKC theta interacting protein, PKCq interacting protein, Thioredoxin like protein 2, TXNL2, TXNL3 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 37-40 kDa |
| GenBank蛋白编号 | BC014372 |
| 基因名称 | GLRX3 |
| Gene ID (NCBI) | 10539 |
| RRID | AB_2110374 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O76003 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLRX3(Glutaredoxin-3) is also named as PICOT, TXNL2. Mammalian glutaredoxin 3 is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals(PMID:20226171). Glrx3 could take a pivotal role in colon and lung cancer cells during the tumorigenesis, which is expressed in lung and colon cancers consistently and preferentially(PMID:19797004).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GLRX3 antibody 11254-1-AP | Download protocol |
| IHC protocol for GLRX3 antibody 11254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients. | ||
Exp Cell Res TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT.
| ||
Biosci Biotechnol Biochem Identification of proteins that bind to the neuroprotective agent neoechinulin A.
| ||
Haematologica Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. | ||
J Clin Invest CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes |
