验证数据展示
经过测试的应用
| Positive WB detected in | C6 cells, A549 cells, MCF-7 cells, rat brain tissue, rat heart tissue |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human testis tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human testis tissue, mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11303-1-AP targets SERPINE2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SERPINE2 fusion protein Ag1830 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2 |
| 别名 | PI7, PN1, Serpin E2, PI 7, Peptidase inhibitor 7 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 44-50 kDa |
| GenBank蛋白编号 | BC015663 |
| 基因名称 | SERPINE2 |
| Gene ID (NCBI) | 5270 |
| RRID | AB_10732939 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07093 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SERPINE2 antibody 11303-1-AP | Download protocol |
| IHC protocol for SERPINE2 antibody 11303-1-AP | Download protocol |
| IF protocol for SERPINE2 antibody 11303-1-AP | Download protocol |
| IP protocol for SERPINE2 antibody 11303-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Lett SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.
| ||
Respir Res High expression level of serpin peptidase inhibitor clade E member 2 is associated with poor prognosis in lung adenocarcinoma.
| ||
J Immunol Deep Coverage Tissue and Cellular Proteomics Revealed IL-1β Can Independently Induce the Secretion of TNF-Associated Proteins from Human Synoviocytes. | ||
Front Cell Dev Biol BAP31 Promotes Tumor Cell Proliferation by Stabilizing SERPINE2 in Hepatocellular Carcinoma. | ||
J Cell Physiol Transcriptional profiling of endometriosis tissues identifies genes related to organogenesis defects. |
