验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, Jurkat cells, mouse testis tissue, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11324-1-AP targets DDX20 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | DDX20 fusion protein Ag1863 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 |
| 别名 | Gemin 3, EC:3.6.1.15, DP103, DEAD box protein DP 103, DEAD box protein 20 |
| 计算分子量 | 824 aa, 92 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC011556 |
| 基因名称 | DDX20 |
| Gene ID (NCBI) | 11218 |
| RRID | AB_2092401 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHI6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 (DDX20), also known as DP103 or Gemin3, is a member of the DEAD box protein family expressed ubiquitously. DEAD family proteins use energy from ATP hydrolysis for RNA chaperoning and RNPase activity (PMID: 27121695). As a core member of the survival motor neuron (SMN) complex, DDX20 participate in small nuclear ribonucleoprotein (snRNP) biogenesis. Second, DDX20 have direct roles in gene expression in view of its implication in transcription and post-transcriptional gene silencing. Addition, the false expression of DDX20 could have deleterious effects on cellular homeostasis thus leading to cancer development and progression (PMID:29523774). Anymore, DDX20 could be identified as a biomarker and metastasis-driving oncogene of human breast cancer (PMID: 25083991). The detected weight of DDX20 is slightly higher than the theoretical molecular weight that is because of phosphorylation after translation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX20 antibody 11324-1-AP | Download protocol |
| IHC protocol for DDX20 antibody 11324-1-AP | Download protocol |
| IF protocol for DDX20 antibody 11324-1-AP | Download protocol |
| IP protocol for DDX20 antibody 11324-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest DEAD-box helicase DP103 defines metastatic potential of human breast cancers.
| ||
Dev Cell DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice | ||
Aging (Albany NY) An artificial neural network model to diagnose non-obstructive azoospermia based on RNA-binding protein-related genes | ||
Comput Struct Biotechnol J Analysis of the expression, function, prognosis and co-expression genes of DDX20 in gastric cancer. | ||
Arthritis Res Ther Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies. | ||
Biosci Rep FOXL2 Down-regulates Vitellogenin Expression at Mature Stage in Eriocheir sinensis. |
