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验证数据展示

  • WB analysis of human colon using 11376-1-AP

    WB analysis of human colon using 11376-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 11376-1-AP (SULT1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11376-1-AP

    IHC staining of human breast cancer using 11376-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11376-1-AP (SULT1A4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11376-1-AP

    IHC staining of human breast cancer using 11376-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11376-1-AP (SULT1A4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 11376-1-AP

    IF Staining of HepG2 using 11376-1-AP

    Immunofluorescent analysis of HepG2 cells using 11376-1-AP (SULT1A4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SULT1A4 Polyclonal antibody

SULT1A4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11376-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Monoamine-sulfating phenol sulfotransferase, M PST, HAST3, EC:2.8.2.1, Catecholamine-sulfating phenol sulfotransferase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman colon tissue, BxPC-3 cells, HepG2 cells, human pancreas tissue, mouse pancreas tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11376-1-AP targets SULT1A4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SULT1A4 fusion protein Ag1935 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sulfotransferase family, cytosolic, 1A, phenol-preferring, member 4
别名 Monoamine-sulfating phenol sulfotransferase, M PST, HAST3, EC:2.8.2.1, Catecholamine-sulfating phenol sulfotransferase
计算分子量 295 aa, 34 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC014471
基因名称 SULT1A4
Gene ID (NCBI) 445329
RRIDAB_2197610
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP0DMN0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SULT1A4 antibody 11376-1-APDownload protocol
IHC protocol for SULT1A4 antibody 11376-1-APDownload protocol
IF protocol for SULT1A4 antibody 11376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols