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验证数据展示

  • WB analysis using 11410-1-AP

    WB analysis using 11410-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11410-1-AP (NAT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 11410-1-AP

    IHC staining of human small intestine using 11410-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens).

  • FC experiment of MCF-7 using 11410-1-AP

    FC experiment of MCF-7 using 11410-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug NAT2 Polyclonal antibody (11410-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human breast cancer using 11410-1-AP

    IHC staining of human breast cancer using 11410-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11410-1-AP

    IHC staining of human breast cancer using 11410-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11410-1-AP (NAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NAT2 Polyclonal antibody

NAT2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11410-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

N acetyltransferase type 2, EC:2.3.1.5, EC:2.3.1.118, Arylamine N-acetyltransferase 2, Arylamide acetylase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat liver tissue
Positive IHC detected inhuman small intestine tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11410-1-AP targets NAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NAT2 fusion protein Ag1953 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-acetyltransferase 2 (arylamine N-acetyltransferase)
别名 N acetyltransferase type 2, EC:2.3.1.5, EC:2.3.1.118, Arylamine N-acetyltransferase 2, Arylamide acetylase 2
计算分子量 33.5 kDa
观测分子量38 kDa
GenBank蛋白编号BC015878
基因名称 NAT2
Gene ID (NCBI) 10
RRIDAB_3085371
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11245
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAT2 (Arylamine N-acetyltransferase 2) is well-known for its role in phase II metabolism of xenobiotics and drugs (PMID:36156098). NAT2 and its isozyme, arylamine N-acetyltransferase 1 (NAT1), play key roles in the detoxification of carcinogenic arylamines and xenobiotics by catalyzing acetyl-coenzyme A (CoA)-dependent biotransformation of arylamines to N-arylacetamides. Endogenous NAT2 expression is limited to the liver and small and large intestines, suggesting that NAT2 plays organ-specific roles (PMID:24467436).

实验方案

Product Specific Protocols
WB protocol for NAT2 antibody 11410-1-APDownload protocol
IHC protocol for NAT2 antibody 11410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols