验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human placenta tissue, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11580-1-AP targets TNS4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TNS4 fusion protein Ag2169 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tensin 4 |
| 别名 | Tensin-4, tensin 4, PP14434, C-terminal tensin-like protein, CTEN |
| 计算分子量 | 715 aa, 77 kDa |
| 观测分子量 | 77 kDa |
| GenBank蛋白编号 | BC018706 |
| 基因名称 | TNS4 |
| Gene ID (NCBI) | 84951 |
| RRID | AB_10640582 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IZW8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNS4 (Tensin-4), also known as COOH-terminus tensin-like protein, is a distinctive member with the smallest molecular mass and lacks the N-terminal common region found in other family members. TNS4 plays a crucial role in CRC tumorigenesis, and that suppression of TNS4 would be an effective therapeutic strategy in treating a subset of cetuximab-refractory CRC patients including KRAS activating mutations (PMID: 31409052). TNS4 has been implicated in numerous vital biological processes, including proliferation, migration, cell adhesion, and apoptosis (PMID: 38164166).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TNS4 antibody 11580-1-AP | Download protocol |
| IHC protocol for TNS4 antibody 11580-1-AP | Download protocol |
| IF protocol for TNS4 antibody 11580-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Bioeng Biotechnol Smarce1 and Tensin 4 Are Putative Modulators of Corneoscleral Stiffness. | ||
Oncol Lett Tensin 4 facilitates aerobic glycolysis, migration and invasion of colorectal cancer cells through the β‑catenin/c‑Myc signaling pathway
| ||
Pathol Res Pract Tensin4 promotes invasion and migration of gastric cancer cells via regulating AKT/GSK-3β/snail signaling pathway.
| ||
Antioxid Redox Signal A novel lncRNA FUAT1/TNS4 axis confers chemoresistance by suppressing ROS-mediated apoptosis in gastric cancer | ||
Int J Biol Sci Hypoxia-driven TNS4 fosters HNSCC tumorigenesis by stabilizing integrin α5β1 complex and triggering FAK-mediated Akt and TGFβ signaling pathways |
