验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
11888-1-AP targets XLF in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | XLF fusion protein Ag2496 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nonhomologous end-joining factor 1 |
| 别名 | NHEJ1, Protein cernunnos, XLF, XRCC4 like factor |
| 计算分子量 | 35 kDa |
| 观测分子量 | 33-38 kDa |
| GenBank蛋白编号 | BC030986 |
| 基因名称 | XLF |
| Gene ID (NCBI) | 79840 |
| RRID | AB_2282851 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H9Q4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for XLF antibody 11888-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Med Genet Haploinsufficiency in non-homologous end joining factor 1 induces ovarian dysfunction in humans and mice. | ||
PLoS One 5-Azacytidine Enhances the Radiosensitivity of CNE2 and SUNE1 Cells In Vitro and In Vivo Possibly by Altering DNA Methylation. | ||
Int J Biochem Cell Biol Targeting Ku86 enhances X-ray-induced radiotherapy sensitivity in serous ovarian cancer cells. | ||
FEBS Lett RNase L facilitates the repair of DNA double-strand breaks through the nonhomologous end-joining pathway. |