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POP1 Polyclonal antibody

POP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat no : 12029-1-AP

Print datasheet

Synonyms

验证数据展示

WB analysis using 12029-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12029-1-AP (POP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 12029-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POP1 antibody (12029-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
WB analysis of HeLa using 12029-1-AP
WB result of POP1 antibody (12029-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POP1 transfected HeLa cells.
IP experiment of HeLa using 12029-1-AP
IP result of anti-POP1 (IP:12029-1-AP, 4ug; Detection:12029-1-AP 1:8000) with HeLa cells lysate 1560 ug.
IHC staining of human ovary cancer using 12029-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12029-1-AP (POP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 12029-1-AP

Size

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经过测试的应用

Positive WB detected in	HeLa cells, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 1 publications below
IP	See 1 publications below
RIP	See 1 publications below

产品信息

12029-1-AP targets POP1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IP, RIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Immunogen	POP1 fusion protein Ag2660 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae)
Synonyms
Calculated Molecular Weight	1024 aa, 115 kDa
Observed Molecular Weight	115 kDa
GenBank Accession Number	BC011529
Gene Symbol	POP1
Gene ID (NCBI)	10940
RRID	AB_2166477
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99575
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Processing of Precursor 1 (POP1), a core component of RNase subunits, is an autoantigen in patients with connective tissue disease and is involved in suppressing inflammation.

实验方案

Product Specific Protocols
WB protocol for POP1 antibody 12029-1-AP	Download protocol
IHC protocol for POP1 antibody 12029-1-AP	Download protocol
IF protocol for POP1 antibody 12029-1-AP	Download protocol
IP protocol for POP1 antibody 12029-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Mol Cell HSP70 binds to specific non-coding RNA and regulates human RNA polymerase III Authors - Sergio Leone View Article
human	WB	Mol Cell Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex. Authors - Ok Hyun Park View Article
human	WB	Nat Microbiol The SARS-CoV-2 RNA-protein interactome in infected human cells. Authors - Nora Schmidt View Article
human	WB,RIP,IHC	Research (Wash D C) POP1 Facilitates Proliferation in Triple-Negative Breast Cancer via m6A-Dependent Degradation of CDKN1A mRNA Authors - Chao Zhang View Article
human	WB	Cell Rep UPF1 promotes rapid degradation of m6A-containing RNAs. Authors - Sung Ho Boo View Article
human	IP,WB	Cell Rep m1A and m6A modifications function cooperatively to facilitate rapid mRNA degradation Authors - Sung Ho Boo KD Validated View Article

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POP1 Polyclonal antibody

POP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

WB analysis using 12029-1-AP

IF Staining of HepG2 using 12029-1-AP

WB analysis of HeLa using 12029-1-AP

IP experiment of HeLa using 12029-1-AP

IHC staining of human ovary cancer using 12029-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

HSP70 binds to specific non-coding RNA and regulates human RNA polymerase III

Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex.

The SARS-CoV-2 RNA-protein interactome in infected human cells.

POP1 Facilitates Proliferation in Triple-Negative Breast Cancer via m6A-Dependent Degradation of CDKN1A mRNA

UPF1 promotes rapid degradation of m6A-containing RNAs.

m1A and m6A modifications function cooperatively to facilitate rapid mRNA degradation