验证数据展示
经过测试的应用
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12176-1-AP targets FAM107A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | FAM107A fusion protein Ag2820 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 107, member A |
| 别名 | TU3A, DRR1, Down-regulated in renal cell carcinoma 1 |
| 计算分子量 | 144 aa, 17 kDa |
| GenBank蛋白编号 | BC010561 |
| 基因名称 | FAM107A |
| Gene ID (NCBI) | 11170 |
| RRID | AB_2877832 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95990 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAM107A, also named as TU3A, encodes a 144 amino-acid protein, which contains a coiled-coil domain and a nuclear localization signal. FAM107A as a transcription factor regulates some gene transcription and signal transduction in cell. With the exception of peripheral blood cells, FAM107A is widely expressed in normal tissues, but shows significant loss of expression in renal cell carcinomas and frequent loss of expression in cervical, gastric, ovarian and non small cell lung cancers. Transfection of FAM107A mRNA into cancer cell line inhibits cell growth and proliferation, supporting the evidence that the gene functions as an important tumor suppressor.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM107A antibody 12176-1-AP | Download protocol |
| IF protocol for FAM107A antibody 12176-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Mol Genet A severe mouse model of spinal muscular atrophy develops early systemic inflammation. | ||
Arch Biochem Biophys Loss of family with sequence similarity 107, member A (FAM107A) induces browning in 3T3-L1 adipocytes.
| ||
Cancers (Basel) FAM107A Inactivation Associated with Promoter Methylation Affects Prostate Cancer Progression through the FAK/PI3K/AKT Pathway | ||
Pathol Res Pract FAM107A as a tumor suppressor in esophageal squamous carcinoma inhibits growth and metastasis |
