验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, mouse kidney tissue, human brain tissue |
| Positive IP detected in | A375 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy DNAJA2 Ready-To-Use IHC Kit. DNAJA2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12236-1-AP targets DNAJA2 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP, CoIP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | DNAJA2 fusion protein Ag2877 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DnaJ (Hsp40) homolog, subfamily A, member 2 |
| 别名 | HIRA interacting protein 4, Dnj3, DNAJ, DJA2, Dj3 |
| 计算分子量 | 417 aa, 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC015809 |
| 基因名称 | DNAJA2 |
| Gene ID (NCBI) | 10294 |
| RRID | AB_2230709 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60884 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNAJA2 antibody 12236-1-AP | Download protocol |
| IF protocol for DNAJA2 antibody 12236-1-AP | Download protocol |
| IP protocol for DNAJA2 antibody 12236-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Cancer Down-regulation of BiP/GRP78 sensitizes resistant prostate cancer cells to gene-therapeutic overexpression of REIC/Dkk-3. | ||
J Biol Chem A cytosolic chaperone complex controls folding and degradation of type III CD38. | ||
Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin | ||
J Biol Chem Quantitative interactome proteomics identifies a proteostasis network for GABAA receptors | ||
Cancer Res N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma |
