验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
12271-1-AP targets UCK1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | UCK1 fusion protein Ag2912 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | uridine-cytidine kinase 1 |
| 别名 | Uridine-cytidine kinase 1, Uridine monophosphokinase 1, uridine cytidine kinase 1, UCK 1, EC:2.7.1.48 |
| 计算分子量 | 277 aa, 31 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC015547 |
| 基因名称 | UCK1 |
| Gene ID (NCBI) | 83549 |
| RRID | AB_11182173 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HA47 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Uridine/cytidine kinase-1 is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP). The deduced 277-amino acid protein has a calculated molecular mass of 31 kDa. UCK1 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UCK1 antibody 12271-1-AP | Download protocol |
| IF protocol for UCK1 antibody 12271-1-AP | Download protocol |
| IP protocol for UCK1 antibody 12271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Cancer Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism. | ||
Blood Adv Reprogramming of pyrimidine nucleotide metabolism supports vigorous cell proliferation of normal and malignant T cells | ||
Nat Metab Glucose limitation protects cancer cells from apoptosis induced by pyrimidine restriction and replication inhibition | ||
Oncogene The protection of UCK2 protein stability by GART maintains pyrimidine salvage synthesis for HCC growth under glucose limitation |
