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验证数据展示

  • WB analysis of K-562 using 12276-1-AP

    WB analysis of K-562 using 12276-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12276-1-AP (FIP1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 12276-1-AP

    IF Staining of MCF-7 using 12276-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FIP1L1 antibody (12276-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis using 12276-1-AP

    WB analysis using 12276-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12276-1-AP (FIP1L1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 12276-1-AP

    IHC staining of human colon cancer using 12276-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12276-1-AP (FIP1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FIP1L1 antibody included
  • IHC staining of human colon cancer using 12276-1-AP

    IHC staining of human colon cancer using 12276-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12276-1-AP (FIP1L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FIP1L1 antibody included

FIP1L1 Polyclonal antibody

FIP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12276-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, Jurkat cells, MOLT-4 cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy FIP1L1 Ready-To-Use IHC Kit. FIP1L1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12276-1-AP targets FIP1L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 FIP1L1 fusion protein Ag2921 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FIP1 like 1 (S. cerevisiae)
别名 FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE
计算分子量 594 aa, 66 kDa
观测分子量 67 kDa
GenBank蛋白编号BC017724
基因名称 FIP1L1
Gene ID (NCBI) 81608
RRIDAB_2231572
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UN15
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).

实验方案

Product Specific Protocols
WB protocol for FIP1L1 antibody 12276-1-APDownload protocol
IHC protocol for FIP1L1 antibody 12276-1-APDownload protocol
IF protocol for FIP1L1 antibody 12276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols