验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue, human brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | Neuro-2a cells |
Planning an IHC experiment? We recommend our IHCeasy SORT1 Ready-To-Use IHC Kit. SORT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12369-1-AP targets Sortilin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Sortilin fusion protein Ag2997 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sortilin 1 |
| 别名 | SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95 |
| 计算分子量 | 831 aa, 92 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | BC023542 |
| 基因名称 | Sortilin |
| Gene ID (NCBI) | 6272 |
| ENSEMBL Gene ID | ENSG00000134243 |
| RRID | AB_2302242 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99523 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Sortilin antibody 12369-1-AP | Download protocol |
| IHC protocol for Sortilin antibody 12369-1-AP | Download protocol |
| IF protocol for Sortilin antibody 12369-1-AP | Download protocol |
| IP protocol for Sortilin antibody 12369-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Evidence for sortilin modulating regional accumulation of human tau prions in transgenic mice. | ||
J Cell Biol CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis. | ||
PLoS Pathog Prions amplify through degradation of the VPS10P sorting receptor sortilin.
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EBioMedicine Neurotensin contributes to pediatric intestinal failure-associated liver disease via regulating intestinal bile acids uptake.
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