• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 12498-1-AP

    WB analysis using 12498-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 12498-1-AP

    WB analysis using 12498-1-AP

    WB result of FOX2/RBM9 antibody (12498-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOX2/RBM9 transfected HEK-293 cells.

  • IHC staining of human breast cancer using 12498-1-AP

    IHC staining of human breast cancer using 12498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOX2/RBM9 antibody included
  • IF Staining of mouse brain using 12498-1-AP

    IF Staining of mouse brain using 12498-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 12498-1-AP

    WB analysis using 12498-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 12498-1-AP

    IP experiment of HepG2 using 12498-1-AP

    IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with HepG2 cells lysate 2680 ug.

  • IP experiment of K-562 using 12498-1-AP

    IP experiment of K-562 using 12498-1-AP

    IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with K-562 cells lysate 1760 ug.

  • IF Staining of HepG2 using 12498-1-AP

    IF Staining of HepG2 using 12498-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOX2/RBM9 antibody (12498-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

FOX2/RBM9 Polyclonal antibody

FOX2/RBM9 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 12498-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)

别名

FOX2, RBM9, HNRBP2, Hexaribonucleotide-binding protein 2, fxh

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293T cells, HepG2 cells, HEK-293 cells, Jurkat cells, NCCIT cells, HeLa cells, Neuro-2a cells
Positive IP detected inHepG2 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue, mouse brain tissue, human ovary tumor tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FOX2/RBM9 Ready-To-Use IHC Kit. FOX2/RBM9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12498-1-AP targets FOX2/RBM9 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse
文献引用反应性human
免疫原 FOX2/RBM9 fusion protein Ag3168 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 9
别名 FOX2, RBM9, HNRBP2, Hexaribonucleotide-binding protein 2, fxh
计算分子量 390 aa, 41 kDa
观测分子量 50 and 60 kDa
GenBank蛋白编号BC013115
基因名称 RBM9
Gene ID (NCBI) 23543
RRIDAB_2877861
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43251
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)

实验方案

Product Specific Protocols
WB protocol for FOX2/RBM9 antibody 12498-1-APDownload protocol
IHC protocol for FOX2/RBM9 antibody 12498-1-APDownload protocol
IF protocol for FOX2/RBM9 antibody 12498-1-APDownload protocol
IP protocol for FOX2/RBM9 antibody 12498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Pharmacol

Integrated pan-cancer analysis reveals the immunological and prognostic potential of RBFOX2 in human tumors

Authors - Fengxian Huang
View Article
humanWB,IF,CoIP

Nat Cell Biol

RBFOX2 recognizes N6-methyladenosine to suppress transcription and block myeloid leukaemia differentiation

Authors - Xiaoyang Dou
View Article
humanWB

iScience

Hypoxia induces hepatocellular carcinoma metastasis via the HIF-1α/METTL16/lnc-CSMD1-7/RBFOX2 axis

Authors - Yingchao Wang
View Article