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NIFK Polyclonal antibody

NIFK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 12615-1-AP

Print datasheet

Synonyms

MKI67IP, Nucleolar protein interacting with the FHA domain of pKI-67, NOPP34, MKI67 FHA domain-interacting nucleolar phosphoprotein, hNIFK

验证数据展示

  • WB analysis using 12615-1-AP

    WB analysis using 12615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12615-1-AP (NIFK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 12615-1-AP

    IF Staining of MCF-7 using 12615-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 12615-1-AP (NIFK antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 12615-1-AP

    IHC staining of human liver cancer using 12615-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12615-1-AP (NIFK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 12615-1-AP

    IHC staining of human liver cancer using 12615-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12615-1-AP (NIFK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of MCF-7 using 12615-1-AP

    FC experiment of MCF-7 using 12615-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug NIFK Polyclonal antibody (12615-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12615-1-AP targets NIFK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen NIFK fusion protein Ag3284 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MKI67 (FHA domain) interacting nucleolar phosphoprotein
Synonyms MKI67IP, Nucleolar protein interacting with the FHA domain of pKI-67, NOPP34, MKI67 FHA domain-interacting nucleolar phosphoprotein, hNIFK
Calculated Molecular Weight 293 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC024238
Gene Symbol NIFK
Gene ID (NCBI) 84365
RRIDAB_2142384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYG3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NIFK antibody 12615-1-APDownload protocol
IHC protocol for NIFK antibody 12615-1-APDownload protocol
IF protocol for NIFK antibody 12615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Authors - Mio Iwasaki
View Article