验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12647-1-AP targets LANCL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | LANCL1 fusion protein Ag3361 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | LanC lantibiotic synthetase component C-like 1 (bacterial) |
| 别名 | LanC-like protein 1, LanC like protein 1, GPR69A, Glutathione S-transferase LANCL1, EC:2.5.1.18 |
| 计算分子量 | 399 aa, 45 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC028685 |
| 基因名称 | LANCL1 |
| Gene ID (NCBI) | 10314 |
| RRID | AB_10638314 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43813 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LanCL1 ( also known as P40 or GRP69A), is homologous to bacterial lanthionine synthetase C (LanC) family, which is involved in the biosynthesis of antimicrobial peptides (lantibiotics). LanCL1 was identified as a reduced glutathione (GSH)-binding protein and primarily expressed in neural tissues and testis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LANCL1 antibody 12647-1-AP | Download protocol |
| IHC protocol for LANCL1 antibody 12647-1-AP | Download protocol |
| IF protocol for LANCL1 antibody 12647-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis LanCL1 protects prostate cancer cells from oxidative stress via suppression of JNK pathway.
| ||
J Lipid Res Lanthionine synthetase C-like protein 2 (LanCL2) is Important for Adipogenic Differentiation.
| ||
Front Microbiol The Endogenous Alterations of the Gut Microbiota and Feces Metabolites Alleviate Oxidative Damage in the Brain of LanCL1 Knockout Mice.
| ||
Exp Neurol LanCL1 protects developing neurons from long-term isoflurane anesthesia-induced neurotoxicity |