验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, A375 cells, fetal HEK-293 cells, HeLa cells, human colon tissue, human kidney tissue, K-562 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human ovary tumor tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12683-1-AP targets MAD2L2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MAD2L2 fusion protein Ag3373 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | MAD2 mitotic arrest deficient-like 2 (yeast) |
| 别名 | hREV7, MAD2 like protein 2, MAD2B, MAD2L2, REV7, REV7 homolog |
| 计算分子量 | 211 aa, 24 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC015244 |
| 基因名称 | MAD2L2 |
| Gene ID (NCBI) | 10459 |
| RRID | AB_2139530 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UI95 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAD2L2 antibody 12683-1-AP | Download protocol |
| IHC protocol for MAD2L2 antibody 12683-1-AP | Download protocol |
| IP protocol for MAD2L2 antibody 12683-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair.
| ||
Nat Commun SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombination. | ||
J Cell Biol Sequestration of CDH1 by MAD2L2 prevents premature APC/C activation prior to anaphase onset.
| ||
Nucleic Acids Res REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ
| ||
Nucleic Acids Res REV1 and polymerase ζ facilitate homologous recombination repair.
| ||
