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验证数据展示

  • WB analysis of MCF-7 using 12689-1-AP

    WB analysis of MCF-7 using 12689-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 12689-1-AP

    IF Staining of A431 using 12689-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 12689-1-AP (DPPA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DPPA2 Polyclonal antibody

DPPA2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12689-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA and More (1)

别名

ECSA, Pluripotent embryonic stem cell-related gene 1 protein, PESCRG1, Developmental pluripotency-associated protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, HepG2 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

12689-1-AP targets DPPA2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human
免疫原 DPPA2 fusion protein Ag3601 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 developmental pluripotency associated 2
别名 ECSA, Pluripotent embryonic stem cell-related gene 1 protein, PESCRG1, Developmental pluripotency-associated protein 2
计算分子量 298 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC018070
基因名称 DPPA2
Gene ID (NCBI) 151871
RRIDAB_2277458
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z7J5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DPPA2 antibody 12689-1-APDownload protocol
IF protocol for DPPA2 antibody 12689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Mol Hum Reprod

Differential expression of the embryo/cancer gene ECSA(DPPA2), the cancer/testis gene BORIS and the pluripotency structural gene OCT4, in human preimplantation development.

Authors - Monk Marilyn M
View Article