验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, human brain tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13023-1-AP targets RRAGB in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | RRAGB fusion protein Ag4116 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Ras-related GTP binding B |
| 别名 | Rag B, RAGB, Ras related GTP binding B, RRAGB |
| 计算分子量 | 346 aa, 40 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC034726 |
| 基因名称 | RRAGB |
| Gene ID (NCBI) | 10325 |
| RRID | AB_2180064 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5VZM2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RRAGB antibody 13023-1-AP | Download protocol |
| IHC protocol for RRAGB antibody 13023-1-AP | Download protocol |
| IP protocol for RRAGB antibody 13023-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop. | ||
J Clin Invest YAP plays a crucial role in the development of cardiomyopathy in lysosomal storage diseases.
| ||
PLoS One Ursolic Acid Inhibits Leucine-Stimulated mTORC1 Signaling by Suppressing mTOR Localization to Lysosome. | ||
Physiol Rep Tissue-specific expression differences in Ras-related GTP-binding proteins in male rats | ||
Cell Signal CircMRP4 orchestrates podocytes injury via the miR-499-5p/RRAGB/mTORC1 axis in diabetic kidney disease | ||
Biochem Biophys Res Commun RRAGB-mediated suppression of PI3K/AKT exerts anti-cancer role in glioblastoma |
