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NKX3-1 Polyclonal antibody

NKX3-1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13069-1-AP

Print datasheet

Synonyms

BAPX2, Homeobox protein Nkx 3.1, NK3 homeobox 1, NKX3, NKX3 1, NKX3.1, NKX3-1, NKX3A

验证数据展示

  • WB analysis of human testis using 13069-1-AP

    WB analysis of human testis using 13069-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 13069-1-AP (NKX3-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 13069-1-AP

    WB analysis of LNCaP using 13069-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 13069-1-AP (NKX3-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 13069-1-AP

    IHC staining of human prostate cancer using 13069-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13069-1-AP (NKX3-1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NKX3.1 antibody included
  • IHC staining of human prostate cancer using 13069-1-AP

    IHC staining of human prostate cancer using 13069-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13069-1-AP (NKX3-1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NKX3.1 antibody included

经过测试的应用

Positive WB detected inLNCaP cells, human testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NKX3.1 Ready-To-Use IHC Kit. NKX3.1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

13069-1-AP targets NKX3-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen NKX3-1 fusion protein Ag4070 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NK3 homeobox 1
Synonyms BAPX2, Homeobox protein Nkx 3.1, NK3 homeobox 1, NKX3, NKX3 1, NKX3.1, NKX3-1, NKX3A
Calculated Molecular Weight 234 aa, 26 kDa
Observed Molecular Weight34-38 kDa
GenBank Accession NumberBC074864
Gene Symbol NKX3-1
Gene ID (NCBI) 4824
RRIDAB_10640580
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99801
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NKX3-1, also named as NKX3.1 and NKX3A, belongs to the NK-3 homeobox family. It is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. NKX3-1 plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells(PMID:19462257). The calcualted molecular weight of NKX3-1 is 26 kDa, but modified NKX3-1 is about 32-35 kDa. (PMID: 18597829)

实验方案

Product Specific Protocols
WB protocol for NKX3-1 antibody 13069-1-APDownload protocol
IHC protocol for NKX3-1 antibody 13069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

bioRxiv

ERG activates a stem-like proliferation-differentiation program in prostate epithelial cells with mixed basal-luminal identity

Authors - Weiran Feng
View Article
mouseIHC

Sci Rep

Prostatic androgen receptor signaling shows an age-related and lobe-specific alteration in mice

Authors - Han Zhang
View Article