验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, LNCaP cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13146-1-AP targets POP5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | POP5 fusion protein Ag3710 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | processing of precursor 5, ribonuclease P/MRP subunit (S. cerevisiae) |
| 别名 | hPop5, HSPC004, POP5, RPP2, RPP20, x0003 |
| 计算分子量 | 163 aa, 19 kDa |
| 观测分子量 | 19 kDa |
| GenBank蛋白编号 | BC012505 |
| 基因名称 | POP5 |
| Gene ID (NCBI) | 51367 |
| RRID | AB_3669153 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q969H6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
POP5 is a component of ribonuclease P and MRP ribonuclease complex that cleaves pre-rRNA sequences and generates mature tRNA molecules. Endogenous hPop5 protein is localized in the nucleus and accumulates in the nucleolus, which is consistent with its association with RNase MRP and RNase P. The human Pop5 cDNA encodes a protein of 163 amino acids with a predicted molecular mass of 18.8 kDa (PMID: 11413139).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for POP5 antibody 13146-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |