验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, A549 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
产品信息
13527-1-AP targets EIF5B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, pig |
| 免疫原 | EIF5B fusion protein Ag4404 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 5B |
| 别名 | Translation initiation factor IF-2, KIAA0741, IF2, eIF-5B, eIF 5B |
| 计算分子量 | 1220 aa, 139 kDa |
| 观测分子量 | 175 kDa |
| GenBank蛋白编号 | BC032639 |
| 基因名称 | EIF5B |
| Gene ID (NCBI) | 9669 |
| RRID | AB_2096200 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60841 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Translation initiation requires the delivery of the initiator methionine tRNA to the 40S ribosomal subunit. The initiator methionine tRNA is delivered by the heterotrimeric complex EIF2 in a ternary complex with GTP that interacts with the 40S subunit. The resulting complex then binds to an mRNA and scans for the AUG start codon. Eukaryotic translation initiation factor 5B (EIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, which functions to general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes, and promotes joining of the 60S ribosomal subunit. A single crossreactive polypeptide of 175 kDa was detected, whereas the predicted size of the protein was 139 kDa. This size discrepancy may be the result of posttranslational modifications of EIF5B or, perhaps more likely, of unusual behavior in SDS-PAGE caused by the highly charged N-terminal region of EIF5B (PMID: 10200264 ).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF5B antibody 13527-1-AP | Download protocol |
| IHC protocol for EIF5B antibody 13527-1-AP | Download protocol |
| IF protocol for EIF5B antibody 13527-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci eIF2A, an initiator tRNA carrier refractory to eIF2α kinases, functions synergistically with eIF5B. | ||
bioRxiv Start codon-associated ribosomal frameshifting mediates nutrient stress adaptation
| ||
bioRxiv LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins
| ||
Sci Adv Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses | ||
J Virol VP3 protein of Senecavirus A promotes viral IRES-driven translation and attenuates innate immunity by specifically relocalizing hnRNPA2B1 |
