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PCDH1 Polyclonal antibody

PCDH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13645-1-AP

Print datasheet

Synonyms

Protocadherin-42, Protocadherin-1, PC42, Cadherin-like protein 1

验证数据展示

  • WB analysis of fetal human brain using 13645-1-AP

    WB analysis of fetal human brain using 13645-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13645-1-AP (PCDH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 13645-1-AP

    IHC staining of human stomach using 13645-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13645-1-AP (PCDH1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13645-1-AP targets PCDH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen PCDH1 fusion protein Ag4591 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protocadherin 1
Synonyms Protocadherin-42, Protocadherin-1, PC42, Cadherin-like protein 1
Calculated Molecular Weight 1060 aa, 115 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC035812
Gene Symbol PCDH1
Gene ID (NCBI) 5097
RRIDAB_2283373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08174
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PCDH1 antibody 13645-1-APDownload protocol
IHC protocol for PCDH1 antibody 13645-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Nanomedicine

VEGF loaded nanofiber membranes inhibit chronic cerebral hypoperfusion-induced cognitive dysfunction by promoting HIF-1a/VEGF mediated angiogenesis

Authors - Yi-Fang Wu
View Article