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验证数据展示

  • WB analysis of SH-SY5Y using 13658-1-AP

    WB analysis of SH-SY5Y using 13658-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13658-1-AP (NR2C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 13658-1-AP

    IHC staining of human prostate cancer using 13658-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13658-1-AP (NR2C1 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

  • IHC staining of human prostate cancer using 13658-1-AP

    IHC staining of human prostate cancer using 13658-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13658-1-AP (NR2C1 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

NR2C1 Polyclonal antibody

NR2C1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13658-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

NR2C1, Orphan nuclear receptor TR2, Testicular receptor 2, TR2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13658-1-AP targets NR2C1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NR2C1 fusion protein Ag4554 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor subfamily 2, group C, member 1
别名 NR2C1, Orphan nuclear receptor TR2, Testicular receptor 2, TR2
计算分子量 467 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC040141
基因名称 NR2C1
Gene ID (NCBI) 7181
RRIDAB_2236290
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13056
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear receptor subfamily 2 group C member 1 (NR2C1), also known as Orphan nuclear receptor TR2, is a member of the steroid/thyroid hormone receptor superfamily that controls a variety of processes, including growth, differentiation and development. TR2 is known to bind to regulatory elements of the erythropoietin gene, the muscle-specific aldolase A gene and the CNTF-15 gene. In addition to TR2, a related orphan receptor, designated TR4, has been identified. TR4 forms heterodimers with TR2, which are thought to be involved in neurogenesis and germ cell development. TR2 is known to be downregulated by both p53 and ionizing radiation, and it may play a role in linking p53 to members of the steroid recepr family.

实验方案

Product Specific Protocols
WB protocol for NR2C1 antibody 13658-1-APDownload protocol
IHC protocol for NR2C1 antibody 13658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols