验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | A2780 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
13722-1-AP targets TTF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | TTF2 fusion protein Ag4671 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcription termination factor, RNA polymerase II |
| 别名 | HuF2, F2, EC:3.6.4.- |
| 计算分子量 | 1162 aa, 129 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC030058 |
| 基因名称 | TTF2 |
| Gene ID (NCBI) | 8458 |
| RRID | AB_2262865 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UNY4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transcription elongation is an key target for control of eukaryotic gene expression. After initiating from a promoter, the RNA polymerase II elongation complex is subjected to a block to elongation by the action of components of N-TEF (negative transcription elongation factor) [PMID: 9748214]. Transcription termination factor 2 (TTF2) is a DsDNA-dependent ATPase that acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. It also contribute to mitotic transcription repression, and involved in pre-mRNA splicing[PMID:15125840, 12927788].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TTF2 antibody 13722-1-AP | Download protocol |
| IHC protocol for TTF2 antibody 13722-1-AP | Download protocol |
| IF protocol for TTF2 antibody 13722-1-AP | Download protocol |
| IP protocol for TTF2 antibody 13722-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell mRNA decapping factors and the exonuclease Xrn2 function in widespread premature termination of RNA polymerase II transcription. | ||
Sci Total Environ PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism. | ||
Nat Commun Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features |
