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NISCH Polyclonal antibody

NISCH Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13813-1-AP

Print datasheet

Synonyms

I1R, I-1 receptor candidate protein, I-1, I 1 receptor candidate protein, I 1

验证数据展示

  • WB analysis using 13813-1-AP

    WB analysis using 13813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13813-1-AP (NISCH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 13813-1-AP

    IP experiment of Jurkat using 13813-1-AP

    IP result of anti-NISCH (IP:13813-1-AP, 4ug; Detection:13813-1-AP 1:500) with Jurkat cells lysate 1560 ug.

  • IP experiment of mouse brain using 13813-1-AP

    IP experiment of mouse brain using 13813-1-AP

    IP result of anti-NISCH (IP:13813-1-AP, 4ug; Detection:13813-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • IHC staining of human stomach using 13813-1-AP

    IHC staining of human stomach using 13813-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13813-1-AP (NISCH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, mouse brain tissue, rat brain tissue
Positive IP detected inJurkat cells, mouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13813-1-AP targets NISCH in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen NISCH fusion protein Ag4880 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nischarin
Synonyms I1R, I-1 receptor candidate protein, I-1, I 1 receptor candidate protein, I 1
Calculated Molecular Weight 1504 aa, 167 kDa
Observed Molecular Weight 190-200 kDa
GenBank Accession NumberBC038102
Gene Symbol NISCH
Gene ID (NCBI) 11188
RRIDAB_10693526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2I1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NISCH antibody 13813-1-APDownload protocol
IHC protocol for NISCH antibody 13813-1-APDownload protocol
IP protocol for NISCH antibody 13813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Anesth Analg

Dexmedetomidine Ameliorates Perioperative Neurocognitive Disorders by Suppressing Monocyte-Derived Macrophages in Mice With Preexisting Traumatic Brain Injury.

Authors - Natsumi Kii
View Article
humanWB,IF

Biochem Biophys Res Commun

Expression of Nischarin negatively correlates with estrogen receptor and alters apoptosis, migration and invasion in human breast cancer.

Authors - Chan Chang
View Article