验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse spleen tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13830-1-AP targets HTR7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | HTR7 fusion protein Ag4742 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled) |
| 别名 | 5 HT 7, 5 HT X, 5 HT7, 5 hydroxytryptamine receptor 7, HTR7, Serotonin receptor 7 |
| 计算分子量 | 445 aa, 49 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC047526 |
| 基因名称 | HTR7 |
| Gene ID (NCBI) | 3363 |
| RRID | AB_2122706 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P34969 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Serotonin (5-hydroxytryptamine; 5-HT) acts as a neurotransmitter and neuromodulator in the central nervous system(CNS) (PMID:23100172). The behavioral effects of 5-HT are mediated by a large family of specific 5-HT receptors. HTR7 (5-hydroxytryptamine receptor 7) is a G protein-coupled receptor (GPCR), and many transcription factors can bind to its promoter, such as AP2, EGR-1, and MAZ (PMID:34164474). It is located in different brain regions, such as the hypothalamus, thalamus, cerebral cortex, and hippocampus (PMID:29637287). HTR7 is associated with circadian rhythm, anxiety, depression, schizophrenia, nociception, and memory (PMID:9154233).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HTR7 antibody 13830-1-AP | Download protocol |
| IHC protocol for HTR7 antibody 13830-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application | ||
Biomedicines T Lymphocyte Serotonin 5-HT7 Receptor Is Dysregulated in Natalizumab-Treated Multiple Sclerosis Patients | ||
Onco Targets Ther 5-HT7 Receptor Contributes to Proliferation, Migration and Invasion in NSCLC Cells. | ||
Cancer Lett Serotonylation in tumor-associated fibroblasts contributes to the tumor-promoting roles of serotonin in colorectal cancer |
