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验证数据展示

  • IHC staining of human breast cancer using 13853-1-AP

    IHC staining of human breast cancer using 13853-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13853-1-AP (DNAJC4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human breast cancer using 13853-1-AP

    IHC staining of human breast cancer using 13853-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13853-1-AP (DNAJC4 antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of MCF-7 using 13853-1-AP

    WB analysis of MCF-7 using 13853-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13853-1-AP (DNAJC4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

DNAJC4 Polyclonal antibody

DNAJC4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13853-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DANJC4, DnaJ like protein HSPF2, DNAJC4, HSPF2, MCG18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13853-1-AP targets DNAJC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DNAJC4 fusion protein Ag4826 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 4
别名 DANJC4, DnaJ like protein HSPF2, DNAJC4, HSPF2, MCG18
计算分子量 28 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC044584
基因名称 DNAJC4
Gene ID (NCBI) 3338
RRIDAB_2095224
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NNZ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DNAJC4 antibody 13853-1-APDownload protocol
IHC protocol for DNAJC4 antibody 13853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols