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验证数据展示

  • WB analysis using 14093-1-AP

    WB analysis using 14093-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14093-1-AP (SNAP91 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 14093-1-AP

    IHC staining of mouse brain using 14093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14093-1-AP (SNAP91 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 14093-1-AP

    IHC staining of mouse brain using 14093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14093-1-AP (SNAP91 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 14093-1-AP

    IF Staining of mouse brain using 14093-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SNAP91 antibody (14093-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SNAP91 Polyclonal antibody

SNAP91 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 14093-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF-P, ELISA

别名

Phosphoprotein F1-20, KIAA0656, Clathrin coat-associated protein AP180, Clathrin coat assembly protein AP180, 91 kDa synaptosomal-associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14093-1-AP targets SNAP91 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性pig
免疫原 SNAP91 fusion protein Ag5224 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptosomal-associated protein, 91kDa homolog (mouse)
别名 Phosphoprotein F1-20, KIAA0656, Clathrin coat-associated protein AP180, Clathrin coat assembly protein AP180, 91 kDa synaptosomal-associated protein
计算分子量92.5 kDa
观测分子量160 kDa
GenBank蛋白编号BC060818
基因名称 SNAP91
Gene ID (NCBI) 9892
RRIDAB_2923560
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60641
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNAP91, also known as AP180 or CALM, is a clathrin-coat assembly protein that sequesters clathrin. SNAP91/AP180 is required for clathrin-dependent endocytosis and SV reformation (PMID: 26903854; PMID: 31843760).The AP180 N-terminal homology (ANTH) domains is crucially involved in membrane budding processes, along with and Epsin N-terminal homology (ENTH) domains (PMID: 29774507).

实验方案

Product Specific Protocols
WB protocol for SNAP91 antibody 14093-1-APDownload protocol
IHC protocol for SNAP91 antibody 14093-1-APDownload protocol
IF protocol for SNAP91 antibody 14093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Emerg Microbes Infect

CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis

Authors - Xin Chen
View Article