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验证数据展示

  • WB analysis using 14106-1-AP

    WB analysis using 14106-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14106-1-AP (NAPB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 14106-1-AP

    IHC staining of human gliomas using 14106-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 14106-1-AP

    IHC staining of human gliomas using 14106-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 14106-1-AP

    IP experiment of mouse brain using 14106-1-AP

    IP result of anti-NAPB (IP:14106-1-AP, 4ug; Detection:14106-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IF Staining of HepG2 using 14106-1-AP

    IF Staining of HepG2 using 14106-1-AP

    Immunofluorescent analysis of HepG2 cells, using NAPB antibody 14106-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

NAPB Polyclonal antibody

NAPB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14106-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

SNAP-beta, SNAPB, SNAP BETA, N-ethylmaleimide-sensitive factor attachment protein beta, Beta-soluble NSF attachment protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14106-1-AP targets NAPB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NAPB fusion protein Ag5239 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-ethylmaleimide-sensitive factor attachment protein, beta
别名 SNAP-beta, SNAPB, SNAP BETA, N-ethylmaleimide-sensitive factor attachment protein beta, Beta-soluble NSF attachment protein
计算分子量 298 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC060840
基因名称 NAPB
Gene ID (NCBI) 63908
RRIDAB_2185010
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H115
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NAPB antibody 14106-1-APDownload protocol
IHC protocol for NAPB antibody 14106-1-APDownload protocol
IF protocol for NAPB antibody 14106-1-APDownload protocol
IP protocol for NAPB antibody 14106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols