验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue |
| Positive IHC detected in | human brain tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14609-1-AP targets SMPD1,ASM in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SMPD1,ASM fusion protein Ag6180 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sphingomyelin phosphodiesterase 1, acid lysosomal |
| 别名 | ASM, SMPD1, SMPD1,ASM |
| 计算分子量 | 70 kDa |
| 观测分子量 | 60 kDa, 70-75 kDa |
| GenBank蛋白编号 | BC041164 |
| 基因名称 | SMPD1 |
| Gene ID (NCBI) | 6609 |
| RRID | AB_2878068 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P17405 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMPD1,ASM antibody 14609-1-AP | Download protocol |
| IHC protocol for SMPD1,ASM antibody 14609-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Host Microbe The β2Integrin Mac-1 Induces Protective LC3-Associated Phagocytosis of Listeria monocytogenes.
| ||
Mol Ther Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase | ||
Acta Neuropathol Commun Inhibition of acid sphingomyelinase reduces reactive astrocyte secretion of mitotoxic extracellular vesicles and improves Alzheimer's disease pathology in the 5xFAD mouse | ||
Cells Novel Isolation Method Reveals Sex-Specific Composition and Neurotoxicity of Small Extracellular Vesicles in a Mouse Model of Alzheimer's Disease | ||
J Mol Med (Berl) Acid sphingomyelinase expression is associated with survival in resectable pancreatic ductal adenocarcinoma |
