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验证数据展示

  • WB analysis of HEK-293 using 14896-1-AP

    WB analysis of HEK-293 using 14896-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14896-1-AP (RRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 14896-1-AP

    WB analysis of HeLa using 14896-1-AP

    WB result of RRP1 antibody (14896-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RRP1 transfected HeLa cells.

  • IF Staining of HeLa using 14896-1-AP

    IF Staining of HeLa using 14896-1-AP

    Immunofluorescent analysis of HeLa cells using 14896-1-AP (RRP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

RRP1 Polyclonal antibody

RRP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14896-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Novel nuclear protein 1, NOP52, NNP-1, NNP1, NNP 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, human spleen tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14896-1-AP targets RRP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 RRP1 fusion protein Ag6686 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal RNA processing 1 homolog (S. cerevisiae)
别名 Novel nuclear protein 1, NOP52, NNP-1, NNP1, NNP 1
计算分子量 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC000380
基因名称 RRP1
Gene ID (NCBI) 8568
RRIDAB_2180625
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56182
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RRP1 antibody 14896-1-APDownload protocol
IF protocol for RRP1 antibody 14896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols