• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 14989-1-AP

    WB analysis using 14989-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14989-1-AP (DARS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 14989-1-AP

    IP experiment of HeLa using 14989-1-AP

    IP result of anti-DARS (IP:14989-1-AP, 4ug; Detection:14989-1-AP 1:5000) with HeLa cells lysate 2160 ug.

  • IHC staining of rat brain using 14989-1-AP

    IHC staining of rat brain using 14989-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14989-1-AP (DARS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DARS1 antibody included

DARS Polyclonal antibody

DARS Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14989-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

别名

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DARS1 Ready-To-Use IHC Kit. DARS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14989-1-AP targets DARS in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 DARS fusion protein Ag6974 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aspartyl-tRNA synthetase
别名 Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS
计算分子量 57 kDa
观测分子量 50-57 kDa
GenBank蛋白编号BC000629
基因名称 DARS
Gene ID (NCBI) 1615
RRIDAB_2261349
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14868
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DARS antibody 14989-1-APDownload protocol
IHC protocol for DARS antibody 14989-1-APDownload protocol
IP protocol for DARS antibody 14989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseCoIP

J Virol

Multiple Host Factors Interact with Hypervariable Domain of Chikungunya Virus nsP3 and Determine Viral Replication in Cell-Specific Mode.

Authors - Chetan D Meshram
View Article
humanWB

Am J Cancer Res

hsa_circ_0000519 promotes the progression of lung adenocarcinoma through the hsa-miR-1296-5p/DARS axis

Authors - Guangxu Tu
View Article