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验证数据展示

  • WB analysis using 15095-1-AP

    WB analysis using 15095-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15095-1-AP (LGMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 15095-1-AP

    IHC staining of mouse kidney using 15095-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15095-1-AP (LGMN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGMN antibody included
  • IHC staining of mouse kidney using 15095-1-AP

    IHC staining of mouse kidney using 15095-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15095-1-AP (LGMN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGMN antibody included

LGMN Polyclonal antibody

LGMN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15095-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

AEP, Asparaginyl endopeptidase, legumain, LGMN, LGMN1, Protease, cysteine 1, PRSC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, HEK-293 cells, HeLa cells, mouse kidney tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LGMN Ready-To-Use IHC Kit. LGMN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15095-1-AP targets LGMN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 LGMN fusion protein Ag7170 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 legumain
别名 AEP, Asparaginyl endopeptidase, legumain, LGMN, LGMN1, Protease, cysteine 1, PRSC1
计算分子量 49 kDa
观测分子量 36 kDa
GenBank蛋白编号BC003061
基因名称 LGMN
Gene ID (NCBI) 5641
RRIDAB_3669198
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99538
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).

实验方案

Product Specific Protocols
WB protocol for LGMN antibody 15095-1-APDownload protocol
IHC protocol for LGMN antibody 15095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols