验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, human testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15509-1-AP targets SPO11 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | SPO11 fusion protein Ag4340 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) |
| 别名 | Cancer/testis antigen 35, CT35, SPO11 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 55-70 kDa |
| GenBank蛋白编号 | BC033591 |
| 基因名称 | SPO11 |
| Gene ID (NCBI) | 23626 |
| RRID | AB_10639508 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5K1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Spo11 is a protein used in a complex along with Mre11 and Rad50 during meiotic recombination. It is also involved in the creation of double stranded breaks in the DNA in the early stages of this process. Several transcript variants encoding different isoforms have been found for this gene.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPO11 antibody 15509-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
