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验证数据展示

  • IP experiment of HeLa using 15654-1-AP

    IP experiment of HeLa using 15654-1-AP

    IP result of anti-ST6GALNAC4 (IP:15654-1-AP, 4ug; Detection:15654-1-AP 1:300) with HeLa cells lysate 980 ug.

ST6GALNAC4 Polyclonal antibody

ST6GALNAC4 Polyclonal Antibody for IP, ELISA
Cat No. 15654-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IP, ELISA

别名

Sialyltransferase 3C, Sialyltransferase 7D, SIAT3 C, SIAT3C, SIAT7 D, SIAT7D, ST6GalNAc IV, ST6GALNAC4, ST6GALNACIV

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15654-1-AP targets ST6GALNAC4 in IP, ELISA applications and shows reactivity with human samples.

经测试应用 IP, ELISA Application Description
经测试反应性 human
免疫原 ST6GALNAC4 fusion protein Ag7995 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4
别名 Sialyltransferase 3C, Sialyltransferase 7D, SIAT3 C, SIAT3C, SIAT7 D, SIAT7D, ST6GalNAc IV, ST6GALNAC4, ST6GALNACIV
计算分子量 34 kDa
观测分子量35 kDa
GenBank蛋白编号BC036705
基因名称 ST6GALNAC4
Gene ID (NCBI) 27090
RRIDAB_3669218
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H4F1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3-N-acetyl-galactosaminide alpha-2,6-sialyltransferase (also known as ST6GALNAC4) is a member of the sialyltransferases, which catalyzes the transfer of sialic acid from cytidine monosphosphate (CMP)-sialic acid to galactose-containing substrates. ST6GALNAC4 may be induced abnormal TGFBR2 glycosylation, resulting in the higher protein levels of TGFBR2 and TGF pathway increased activation (PMID: 37381011, 37034303). ST6GALNAC4 was reported to influence patient prognosis though subverting immunosurveillance in Chronic lymphocytic leukemia.

实验方案

Product Specific Protocols
IP protocol for ST6GALNAC4 antibody 15654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols