验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human heart tissue, Jurkat cells, mouse lung tissue, K-562 cells, U2OS cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
产品信息
15688-1-AP targets ERCC6L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | ERCC6L fusion protein Ag8223 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | excision repair cross-complementing rodent repair deficiency, complementation group 6-like |
| 别名 | Tumor antigen BJ HCC 15, PLK1-interacting checkpoint helicase, PICH, EC:3.6.4.12, DNA excision repair protein ERCC-6-like |
| 计算分子量 | 419 aa, 46 kDa, 141 kDa |
| 观测分子量 | 180 kDa |
| GenBank蛋白编号 | BC008808 |
| 基因名称 | ERCC6L |
| Gene ID (NCBI) | 54821 |
| RRID | AB_2098171 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q2NKX8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERCC6L, also named as Tumor antigen BJ-HCC-15 or PICH, is a 1250 amino acid protein, which contains one helicase ATP-binding domain, one helicase C-terminal domain and two TPR repeats. ERCC6L localizes to kinetochores, inner centromeres and belongs to the SNF2/RAD54 helicase family. ERCC6L as a DNA helicase that acts as an essential component of the spindle assembly checkpoint. The calculated molecular weight of ERCC6L is 140 kDa, but the phosphorylated modification of molecular weight of ERCC6L is about 180kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERCC6L antibody 15688-1-AP | Download protocol |
| IHC protocol for ERCC6L antibody 15688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Eur J Med Res Potential 'anti-cancer' effects of esketamine on proliferation, apoptosis, migration and invasion in esophageal squamous carcinoma cells | ||
J Ethnopharmacol Gypenoside LI arrests the cell cycle of breast cancer in G0/G1 phase by down-regulating E2F1. | ||
Oncol Rep Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma. | ||
BMC Cancer ERCC6L promotes the progression of hepatocellular carcinoma through activating PI3K/AKT and NF-κB signaling pathway.
| ||
Oncol Lett ERCC6L promotes cell growth and invasion in human colorectal cancer.
| ||
Cancer Res PICH activates Cyclin A1 transcription to drive S-phase progression and chemoresistance in gastric cancer |
