验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells, human liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
产品信息
15735-1-AP targets DHRS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | DHRS2 fusion protein Ag8508 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dehydrogenase/reductase (SDR family) member 2 |
| 别名 | DHRS2, Dicarbonyl reductase HEP27, HEP27, Protein D, SDR25C1 |
| 计算分子量 | 300 aa, 32 kDa |
| 观测分子量 | 27-30 kDa |
| GenBank蛋白编号 | BC007339 |
| 基因名称 | DHRS2 |
| Gene ID (NCBI) | 10202 |
| RRID | AB_2091847 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13268 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DHRS2 antibody 15735-1-AP | Download protocol |
| IHC protocol for DHRS2 antibody 15735-1-AP | Download protocol |
| IP protocol for DHRS2 antibody 15735-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Epigenetics Decreased DHRS2 expression is associated with HDACi resistance and poor prognosis in ovarian cancer.
| ||
Transl Oncol STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression | ||
Iran J Public Health Auxiliary Diagnosis and Prognostic Value of Dehydrogenase/Reductase 2 (DHRS2) in Various Tumors |
