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验证数据展示

  • WB analysis using 15757-1-AP

    WB analysis using 15757-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15757-1-AP (C1QL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 15757-1-AP

    IHC staining of mouse cerebellum using 15757-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15757-1-AP (C1QL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 15757-1-AP

    IHC staining of mouse cerebellum using 15757-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15757-1-AP (C1QL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 15757-1-AP

    IF Staining of mouse cerebellum using 15757-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using C1QL1 antibody (15757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

C1QL1 Polyclonal antibody

C1QL1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 15757-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

CRF, Complement component 1 Q subcomponent-like 1, C1QRF, C1q-related factor, C1q/TNF-related protein 14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15757-1-AP targets C1QL1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 C1QL1 fusion protein Ag8348 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component 1, q subcomponent-like 1
别名 CRF, Complement component 1 Q subcomponent-like 1, C1QRF, C1q-related factor, C1q/TNF-related protein 14
计算分子量 258 aa, 26 kDa
观测分子量30 kDa
GenBank蛋白编号BC008798
基因名称 C1QL1
Gene ID (NCBI) 10882
RRIDAB_3085471
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75973
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1QL1 (complement component 1, q subcomponent-like 1) is one of the C1q/tumor-necrosis-factor-related protein (CTRP) family members. C1QL1 is synthesized and secreted mainly by the brain and adipose tissues. It is implicated in synapse formation or elimination in cultured hippocampal neurons in vitro.

实验方案

Product Specific Protocols
WB protocol for C1QL1 antibody 15757-1-APDownload protocol
IHC protocol for C1QL1 antibody 15757-1-APDownload protocol
IF protocol for C1QL1 antibody 15757-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols