验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293T cells, NIH/3T3 cells, C6 cells, mouse pancreas tissue, HUVEC cells, HepG2 cells |
| Positive IHC detected in | mouse brain tissue, human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
15799-1-AP targets RPL29 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RPL29 fusion protein Ag8510 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L29 |
| 别名 | ribosomal protein L29, Large ribosomal subunit protein eL29, HUMRPL29, HIP, Cell surface heparin-binding protein HIP |
| 计算分子量 | 159 aa, 18 kDa |
| 观测分子量 | 20-25 kDa |
| GenBank蛋白编号 | BC008926 |
| 基因名称 | RPL29 |
| Gene ID (NCBI) | 6159 |
| RRID | AB_2878187 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P47914 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomal protein L29 (Rpl29) is a component of the large (60S) ribosomal subunit which is abundantly expressed in all cell types, plays a regulatory role in translation efficiency and is essential for protein synthesis (PMID: 17195189). In contrast to adult tissues, RPL29 displays a much more widespread pattern of expression throughout embryonic development, and high levels of RPL29 are found in all developing organs. Several reports showed that RPL29 expression is up-regulated in human carcinomas and constitutes a candidate marker for abnormal growth when overexpressed(PMID: 9788632, 10383165).The antibody can detect the band around 22kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL29 antibody 15799-1-AP | Download protocol |
| IHC protocol for RPL29 antibody 15799-1-AP | Download protocol |
| IF protocol for RPL29 antibody 15799-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Sci Germline Deletion of Huntingtin Causes Male Infertility and Arrested Spermiogenesis in Mice. | ||
Sci Rep The tissue specific regulation of miR22 expression in the lung and brain by ribosomal protein L29. | ||
Nucleic Acids Res ADAR3 modulates neuronal differentiation and regulates mRNA stability and translation |
