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  • Featured Product
  • KD/KO Validated

PKM2-specific Polyclonal antibody

PKM2-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 15822-1-AP

Print datasheet

Synonyms

PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM2

验证数据展示

  • WB analysis of HEK-293 cells using 15822-1-AP

    WB analysis of HEK-293 cells using 15822-1-AP

    WB result of PKM2 specific antibody (15822-1-AP, 1:5000) with si-control and si-PKM2 transfected HEK293 cells.

  • WB analysis using 15822-1-AP

    WB analysis using 15822-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15822-1-AP

    WB analysis using 15822-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 15822-1-AP

    IHC staining of human lung cancer using 15822-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with PKM2 antibody included
  • IHC staining of human lung cancer using 15822-1-AP

    IHC staining of human lung cancer using 15822-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with PKM2 antibody included
  • IP experiment of A549 using 15822-1-AP

    IP experiment of A549 using 15822-1-AP

    IP result of anti-PKM2-specific (IP:15822-1-AP, 4ug; Detection:15822-1-AP 1:4000) with A549 cells lysate 920 ug.

  • IP experiment of MCF-7 using 15822-1-AP

    IP experiment of MCF-7 using 15822-1-AP

    IP result of anti-PKM2-specific (IP:15822-1-AP, 4ug; Detection:15822-1-AP 1:2000) with MCF-7 cells lysate 1880 ug.

  • IHC staining of human colon using 15822-1-AP

    IHC staining of human colon using 15822-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKM2 antibody included
  • IHC staining of human lung cancer using 15822-1-AP

    IHC staining of human lung cancer using 15822-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKM2 antibody included
  • IHC staining of human colon cancer using 15822-1-AP

    IHC staining of human colon cancer using 15822-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKM2 antibody included
  • IF Staining of MCF-7 using 15822-1-AP

    IF Staining of MCF-7 using 15822-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PKM2-specific antibody (15822-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 15822-1-AP

    IF Staining of HeLa using 15822-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (15822-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, 293 cell, C6 cells, HEK-293 cells, HeLa cells, mouse brain tissue, N/A, Recombinant protein, HEK-293T cells, MCF-7 cells, mouse stomach tissue
Positive IP detected inA549 cells, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PKM2 Ready-To-Use IHC Kit. PKM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15822-1-AP targets PKM2-specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, bovine
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PKM pyruvate kinase, muscle
Synonyms PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM2
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberNM_002654
Gene Symbol PKM
Gene ID (NCBI) 5315
RRIDAB_1851537
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14618
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

实验方案

Product Specific Protocols
WB protocol for PKM2-specific antibody 15822-1-APDownload protocol
IHC protocol for PKM2-specific antibody 15822-1-APDownload protocol
IF protocol for PKM2-specific antibody 15822-1-APDownload protocol
IP protocol for PKM2-specific antibody 15822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Nature

The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.

Authors - Christofk Heather R HR
  • KD Validated
View Article
mouseWB

Cell Metab

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Authors - Qinqin He
View Article
humanWB,IP

Mol Cell

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Authors - Youming Zhu
View Article
humanWB

Mol Cell

A Peptide Encoded by a Putative lncRNA HOXB-AS3 Suppresses Colon Cancer Growth.

Authors - Jin-Zhou Huang
View Article
human,mouseWB,IHC

Biomaterials

PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy

Authors - Lu Wang
View Article
humanIP

Nat Commun

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Authors - Chunqing Wang
View Article