验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue |
| Positive IHC detected in | human placenta tissue, human kidney tissue, human liver tissue, human lung tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy CPA1 Ready-To-Use IHC Kit. CPA1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 5 publications below |
产品信息
15836-1-AP targets Carboxypeptidase A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 | Carboxypeptidase A1 fusion protein Ag8586 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | carboxypeptidase A1 (pancreatic) |
| 别名 | CPA1, EC:3.4.17.1, CPA |
| 计算分子量 | 419 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC005279 |
| 基因名称 | Carboxypeptidase A1 |
| Gene ID (NCBI) | 1357 |
| RRID | AB_2085586 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P15085 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Carboxypeptidase A1 antibody 15836-1-AP | Download protocol |
| IHC protocol for Carboxypeptidase A1 antibody 15836-1-AP | Download protocol |
| IF protocol for Carboxypeptidase A1 antibody 15836-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep αE-Catenin Is a Positive Regulator of Pancreatic Islet Cell Lineage Differentiation. | ||
Development Focal adhesion kinase-mediated signaling controls the onset of pancreatic cell differentiation | ||
J Nutr Biochem Intermittent protein restriction improves glucose homeostasis in Zucker diabetic fatty rats and single-cell sequencing reveals distinct changes in β cells | ||
Pancreatology Smad4 deficiency substitutes Cdkn2b but not Cdkn2a downregulation in pancreatic cancer following induction of genetic events in adult mice. | ||
Dis Model Mech A novel pancreatic cancer model originated from transformation of acinar cells in adult tree shrew, a primate-like animal. |
