验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse spleen tissue, mouse testis tissue, HepG2 cells, MCF-7 cells, mouse kidney tissue |
| Positive IHC detected in | human cervical cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16107-1-AP targets HSF1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | HSF1 fusion protein Ag9023 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heat shock transcription factor 1 |
| 别名 | Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1 |
| 计算分子量 | 529 aa, 57 kDa |
| 观测分子量 | 68-80 kDa |
| GenBank蛋白编号 | BC014638 |
| 基因名称 | HSF1 |
| Gene ID (NCBI) | 3297 |
| RRID | AB_11182610 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q00613 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSF1 antibody 16107-1-AP | Download protocol |
| IHC protocol for HSF1 antibody 16107-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Commun RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases. | ||
Am J Physiol Cell Physiol Cancer associated fibroblasts suppressed ferroptosis in glioblastoma via upregulating lncRNA DLEU1 | ||
Ann Thorac Surg Upregulation of Nuclear Heat Shock Factor 1 Contributes to Tumor Angiogenesis and Poor Survival in Patients With Non-Small Cell Lung Cancer. | ||
Front Microbiol The Capsid Protein VP1 of Coxsackievirus B Induces Cell Cycle Arrest by Up-Regulating Heat Shock Protein 70. | ||
Pharm Biol Aspirin relieves the calcification of aortic smooth muscle cells by enhancing the heat shock response. | ||
Toxicol In Vitro Ginsenoside Rg3 induces FUT4-mediated apoptosis in H. pylori CagA-treated gastric cancer cells by regulating SP1 and HSF1 expressions. |
