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RPL10L Polyclonal antibody

RPL10L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16222-1-AP

Print datasheet

Synonyms

60S ribosomal protein L10 like, ribosomal protein L10 like, RPL10_5_1358, RPL10L

验证数据展示

  • WB analysis using 16222-1-AP

    WB analysis using 16222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16222-1-AP (RPL10L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16222-1-AP

    WB analysis using 16222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16222-1-AP (RPL10L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells, SKOV-3 cells, mouse testis tissue, rat testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16222-1-AP targets RPL10L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RPL10L fusion protein Ag9275 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L10-like
Synonyms 60S ribosomal protein L10 like, ribosomal protein L10 like, RPL10_5_1358, RPL10L
Calculated Molecular Weight 214 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC014310
Gene Symbol RPL10L
Gene ID (NCBI) 140801
RRIDAB_3085482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96L21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPL10L (Ribosomal Protein L10 Like), is a testis-specific component of the ribosome, is responsible for regulating the biosynthesis and folding of a subset of male germ-cell-specific proteins that are essential for the formation of sperm(Uniprot). RPL10L plays an essential role during meiosis of spermatogenesis by compensating for its X-linked parental paralog, RPL10, during and after meiotic sex chromosome inactivation(MSCI) (PMID: 28502657).

实验方案

Product Specific Protocols
WB protocol for RPL10L antibody 16222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols