验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
16812-1-AP targets LMF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | LMF1 fusion protein Ag10349 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lipase maturation factor 1 |
| 别名 | C16orf26, HMFN1876, JFP11, lipase maturation factor 1, LMF1, TMEM112, TMEM112A, Transmembrane protein 112 |
| 计算分子量 | 567 aa, 65 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC010738 |
| 基因名称 | LMF1 |
| Gene ID (NCBI) | 64788 |
| RRID | AB_2878320 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96S06 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LMF1, also named as C16orf26 and TMEM112, is involved in the maturation of lipoprotein lipase (LPL) and hepatic lipase in endoplasmic reticulum. It is required for maturation and transport of active lipoprotein lipase (LPL) through the secretory pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LMF1 antibody 16812-1-AP | Download protocol |
| IHC protocol for LMF1 antibody 16812-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |