验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, Jurkat cells, SH-SY5Y cells, MCF-7 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16993-1-AP targets MGAT5B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, rat, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 | MGAT5B fusion protein Ag10678 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B |
| 别名 | GlcNAc T Vb, GnT IX, GnT VB, hGnTVb, KIAA2008, MGAT5B |
| 计算分子量 | 394 aa, 44 kDa, 89 kDa |
| 观测分子量 | 89-100 kDa |
| GenBank蛋白编号 | BC062354 |
| 基因名称 | MGAT5B |
| Gene ID (NCBI) | 146664 |
| RRID | AB_10858919 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q3V5L5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MGAT5B antibody 16993-1-AP | Download protocol |
| IHC protocol for MGAT5B antibody 16993-1-AP | Download protocol |
| IF protocol for MGAT5B antibody 16993-1-AP | Download protocol |
| IP protocol for MGAT5B antibody 16993-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Schizophr Res Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia. | ||
Medicine (Baltimore) Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma | ||
J Biol Chem Brain-specific glycosylation enzyme GnT-IX maintains levels of protein tyrosine phosphatase receptor PTPRZ, thereby mediating glioma growth |
