验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human skeletal muscle tissue, HepG2 cells，mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17032-1-AP targets GRK5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | GRK5 fusion protein Ag10511 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor kinase 5 |
| 别名 | GPRK5, GRK5 |
| 计算分子量 | 590 aa, 68 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC064506 |
| 基因名称 | GRK5 |
| Gene ID (NCBI) | 2869 |
| RRID | AB_2115453 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P34947 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GRK5, also named as GPRK5, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. It is Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. GRK5 is crucial for proper neuronal morphogenesis and the establishment of functional neuronal circuitry.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GRK5 antibody 17032-1-AP | Download protocol |
| IHC protocol for GRK5 antibody 17032-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomaterials Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure | ||
Arthritis Rheumatol GRK5 Inhibition Attenuates Cartilage Degradation via Decreased NF-κB Signaling.
| ||
Cell Rep METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling | ||
Front Mol Biosci Co-Overexpression of GRK5/ACTC1 Correlates With the Clinical Parameters and Poor Prognosis of Epithelial Ovarian Cancer. | ||
Biochim Biophys Acta Mol Cell Res Ligand-dependent internalization of Bombyx mori tachykinin-related peptide receptor is regulated by PKC, GRK5 and β-arrestin2/BmKurtz. | ||
Sci Rep G protein-coupled receptor kinase 5 deletion suppresses synovial inflammation in a murine model of collagen antibody-induced arthritis. |
