验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse lung tissue, mouse brain tissue, Jurkat cells |
| Positive IP detected in | HeLa cells, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17170-1-AP targets IARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | IARS2 fusion protein Ag10696 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | isoleucyl-tRNA synthetase 2, mitochondrial |
| 别名 | IARS2, IleRS, Isoleucine tRNA ligase |
| 计算分子量 | 1012 aa, 114 kDa |
| 观测分子量 | 114 kDa |
| GenBank蛋白编号 | BC010218 |
| 基因名称 | IARS2 |
| Gene ID (NCBI) | 55699 |
| RRID | AB_2264448 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NSE4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IARS2 antibody 17170-1-AP | Download protocol |
| IHC protocol for IARS2 antibody 17170-1-AP | Download protocol |
| IF protocol for IARS2 antibody 17170-1-AP | Download protocol |
| IP protocol for IARS2 antibody 17170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation. | ||
Discov Oncol High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer | ||
Rev Assoc Med Bras (1992) IARS2 regulates proliferation, migration, and angiogenesis of human umbilical vein endothelial cells. | ||
Nat Commun Targeted degradation of extracellular mitochondrial aspartyl-tRNA synthetase modulates immune responses | ||
Orphanet J Rare Dis IARS2 mutations lead to Leigh syndrome with a combined oxidative phosphorylation deficiency | ||
Front Oncol The Oncogene IARS2 Promotes Non-small Cell Lung Cancer Tumorigenesis by Activating the AKT/MTOR Pathway.
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